DNASTAR - Cloning and Primer Design in Lasergene Core Suite - Duration: 56:59. DNASTARInc 5,584 views. DNASTAR - Getting started with the Lasergene instant free trial - Duration: 2:31.
To install Lasergene as a standalone product: Log in to your DNASTAR account and go to the My Licenses page. Press the appropriate button (Lasergene for Windows, Lasergene for Mac OS X) to download the standalone installer.Run the installer, following the instructions on each screen.
DNASTAR is a global software company that has been meeting the needs of life scientists for over 25 years by providing innovative and easy to use software fo.
DNAStar Lasergene. Lasergene is comprehensive software for DNA and protein sequence analysis, contig assembly, and sequence project management with an expanded next-generation sequencing assembly capability. All Cincinnati Children's.

Dynastar Manual
Dnastar Manual

See how to align Sanger sequencing data against a reference sequence in SeqMan Pro. After assembly, you can use SeqMan Pro's analysis and visualization tools.

The Department of Microbiology & Immunology has a 100 user DNASTAR Lasergene license for departmental use. To use the software, follow these steps:

1) Download the appropriate version of the DNASTAR Lasergene software installer to your computer:

Macintosh
DNASTAR Lasergene 14.1 Installer for Macintosh OS X 10.10 or higher (680 MB .dmg)

Windows
DNASTAR Lasergene 14.1 Installer for Windows 7 64 bit or higher (613 MB .exe)

For older operating systems:

Older Macintosh Versions
DNASTAR Lasergene 12.1 Installer for Macintosh OS X 10.7 or higher (904 MB .dmg)
DNASTAR Lasergene 11.2 Installer for Macintosh OS X 10.6 (354 MB .dmg)
DNASTAR Lasergene 9.0 Installer for Macintosh OS X 10.5 (397 MB .dmg)
Older Windows Versions
DNASTAR Lasergene 12.1 Installer for Windows 7 64 bit or higher (697 MB .exe)
DNASTAR Lasergene 9.0 Installer for Windows XP, Vista or 7 (342 MB .exe)

Please make sure that Java is fully updated from Software Update (Mac) or WIndows Update (Windows).

2) Run the downloaded DNASTAR Lasergene installer on your computer. You only need to install the Lasergene Core Suite (and optionally DNASTAR Navigator - we have not licensed SeqMan or GenVision). During the installation process, the software will ask for a license authorization key. At that point, select 'Network' for DNASTAR License Type and license server number your lab was provided with. Your computer must have access to the Columbia network for DNASTAR Lasergene to function, as the software requires a network license server. If you need to use the software from an off campus location install and use VPN software. If you have any other questions about using DNASTAR Lasergene, or need license server information, contact the system administrator.

3) The following DNASTAR Lasergene tutorials and tutorial data may be helpful:

DNASTAR Lasergene Tutorials (online only) and DNASTAR Tutorial Data for Macintosh (413 MB .zip) or DNASTAR Tutorial Data for Windows (393 MB .zip)

DNASTAR Lasergene 11 Tutorial (6.4 MB .pdf)

DNASTAR Lasergene 9 Tutorial (5.7 MB .pdf) and DNASTAR 9 Tutorial Data for Macintosh (77.3 MB .zip) or DNASTAR 9 Tutorial Data for Windows (77.4 MB .zip)

The easiest way to plan, visualize and document your everyday molecular cloning procedures

Improve accuracy while moving faster, so you save time and money.

Elegant, information-rich windows for simulating common cloning and PCR methods
Clear visual schematics let you see exactly how your construct will be put together
SnapGene helps you identify and avoid common mis-steps by keeping track of details like DNA methylation and phosphorylation

With unparalleled visibility and control, even novice cloners will quickly accomplish complex tasks.

Gain unparalleled visibility of your DNA or protein sequence
Create and browse richly annotated plasmid maps or scan large DNA sequences with thousands of annotated features
Confirm your cloning results with highly visual alignment of sequencing results to your simulated construct

Automatic documentation of simulated procedures saves time and makes sharing and saving work easier, more efficient, and more effective.

Automatically generate a rich graphical history of every sequence edit and cloning procedure
Enhance collaboration and retain knowledge by sharing constructs complete with the historical details of how it was made
Experiment confidently with comprehensive “undo” capabilities that retrace your steps

Dynastar Manual

I just want to say that I LOVE SnapGene. It has dramatically altered the research that my lab does, giving us the confidence to take on difficult cloning with minimal effort!

Beyond the Basics of Molecular Cloning

SnapGene is the most popular cloning tool for a reason. It’s fast, smart and extremely user-friendly

Intuitive technology identifies design flaws in cloning procedures so they can be corrected
Simulate standard PCR using your own primers, or allow SnapGene to design them automatically
Specialised cloning tools ensure fast accurate construct design for all major molecular cloning techniques

SnapGene is the most intuitive, easy to use cloning program I have ever been able to get my hands on.

Verify your project design and prevent errors

Dnastar Manual

Automatically View Plasmid Features

Annotate features on your plasmids using SnapGene’s curated feature database or your own custom features
Display enzyme sites, features, primers, ORFs, translations and more on plasmid maps or in detail on the sequence view
Customize your maps with flexible annotation and visualization controls

Check Your Sequences with Alignment Tools

Validate your sequenced constructs match your simulated construct using the powerful Align to Reference tool
Align sequences using trusted algorithms for pairwise and multiple alignments, including Clustal Omega, MAFFT, MUSCLE and T-Coffee
Assemble Sanger sequencing reads into complete contigs using CAP3

Realistic Agarose Gel Simulations

Visualise exactly what you will see in the lab with SnapGene’s empirically based gel simulation algorithm
Flexible configuration of all gel elements, including number of lanes, % agarose, running time and a full set of MW markers
Record and identify your band of interest with detailed fragment information for each lane

Beautiful graphic interface especially for marking features and multiple sequence alignments. THANK YOU!!

Configured for automatic documentation and easy data exchange

Automatically Create a Graphical History

Record experimental procedures and changes to documents visually
Show history colors in history, map and sequence views
Fully resurrectable sequence history embedded in the document

Convert and Share Data

Store, search, share and organize your sequences, files and maps
Work collaboratively with sequences in collections stored on sharable drives, servers or cloud services
Easily read and export sequences, annotations and other metadata from many common file formats

The history feature (my favorite) keeps track of each step, and any construct I get from colleagues comes complete with how it was made. I recommend this software to anyone who clones DNA.

All Features

Explore a list of SnapGene’s robust set of features designed to enhance your everyday
molecular cloning procedures

Molecular Cloning

Restriction Cloning
Gateway Cloning
Gibson Assembly
NEBuilder HiFi Assembly
In-Fusion Cloning
TA & GC Cloning
TOPO Cloning

Primers

Design primers
Anneal two oligos to form a double-stranded product

PCR and Mutagenesis

Simulate PCR
Overlap extension PCR
Primer-directed mutagenesis

Enzyme Sets

Predefined enzyme sets - by company or cutter
Create custom enzyme sets
View detailed enzyme information
Rich support for methylation sensitivity and associated error prevention